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Semi-random barcode design Schematic of semi-random barcode oligonucleotide design with restriction sites used for insertion and a barcode ID tag.

Journal: STAR Protocols

Article Title: Protocol to track single-cell-derived clones using DNA barcoding combined with single-cell RNA sequencing

doi: 10.1016/j.xpro.2025.104229

Figure Lengend Snippet: Semi-random barcode design Schematic of semi-random barcode oligonucleotide design with restriction sites used for insertion and a barcode ID tag.

Article Snippet: Package lentiviruses with HEK293T cells and the barcode library plasmid DNA using the Lipofectamine 3000 kit (Invitrogen) as per manufacturer’s instructions. a.

Techniques: